Reference 2020-581

REF:       2020-581      

Subject:   PCR Test      

    

 

Request:

  1. Confirmation that all reported cases of SARS-COV2 in your Trust have been diagnosed and confirmed by PCR test;

 

  1. If not with a PCR test, provide details of what method was employed to confirm the presence of SARS-COV2;

 

  1. Details of the laboratories performing the PCR tests producing the confirmation of SARS-COV2 on behalf of your Trust;

 

  1. Details of the Ct thesholds currently employed in the PCR tests performed by the labs at 3.;

 

  1. Details of the Ct thresholds used to confirm all reported cases of SARS-COV2 in your Trust between 01/01/2020 and 18/12/2020;

 

  1. Details of the manufacturers of the PCR tests used to confirm the presence of SARS-COV2 in your Trust;

 

  1. In the document “Understanding cycle threshold (Ct) in SARS-CoV-2 RT-PCR” published on the UK government website, page 7 includes the statement “Some commercial RT-PCR techniques are closed ‘black box’ systems whereby the operator cannot observe the reaction in real-time and the result is interpreted by software into a qualitative non-interrogatable positive or negative result.” Please confirm which manufacturers have been engaged that employ such a ‘black box’ method, which labs have used such a manufacturer, and which results confirming the presence of SARS-COV2 in the UK have been based on such techniques;

 

  1. Where such results have been produced using the ‘black box’ method, please describe what further action was taken to confirm the results produced by the ‘black box’ method.

 

Response:

 

Please find information attached.

 

2020-581-FOI Request – PCR Test [173 KB PDF]